rabbit polyclonal anti klf6  (Santa Cruz Biotechnology)

Journal: European journal of cancer (Oxford, England : 1990)

Article Title: Increased alternative splicing of the KLF6 tumor suppressor gene correlates with prognosis and tumor grade in patients with pancreatic cancer

doi: 10.1016/j.ejca.2008.06.030

Figure Lengend Snippet: Correlation of clinical data with mRNA KLF6wt/sp Clinical data and ratio of mRNA KLFwt/sp expression were correlated and statistical analysis was performed using the Spearman test. Additionally, Mann Whitney Test was used to analyze the differences between KLF6 ratio of normal patients and cancer patients.

Article Snippet: The sections were then incubated overnight at 4°C with rabbit polyclonal antibody KLF6 (sc7158, Santa Cruz Biotechnology, Santa Cruz, CA) diluted in 0.05 mol/L Tris-HCL buffer containing 1% bovine serum albumin.

Techniques: Expressing, MANN-WHITNEY

Journal: European journal of cancer (Oxford, England : 1990)

Article Title: Increased alternative splicing of the KLF6 tumor suppressor gene correlates with prognosis and tumor grade in patients with pancreatic cancer

doi: 10.1016/j.ejca.2008.06.030

Figure Lengend Snippet: (A) Normal pancreas, displays KLF6 immunostaining in scattered islet cells, faint staining in ductal cells, but no staining in acinus cells (× 50). (B) Ductal adenocarcinoma of the pancreas, demonstrating scattered cytoplasmatic KLF6 immunoreactivity, with some stromal staining. (× 50). (C) Higher power immunostaining for KLF6 in a separate pancreatic adenocarcinoma demonstrating the cytoplasmic and stromal staining (× 400). Insert showing no immunoreactivity of ductal cancer cells in control tissue.

Article Snippet: The sections were then incubated overnight at 4°C with rabbit polyclonal antibody KLF6 (sc7158, Santa Cruz Biotechnology, Santa Cruz, CA) diluted in 0.05 mol/L Tris-HCL buffer containing 1% bovine serum albumin.

Techniques: Immunostaining, Staining, Control

Journal: European journal of cancer (Oxford, England : 1990)

Article Title: Increased alternative splicing of the KLF6 tumor suppressor gene correlates with prognosis and tumor grade in patients with pancreatic cancer

doi: 10.1016/j.ejca.2008.06.030

Figure Lengend Snippet: Representative Western blots are shown with pancreatic cancer at the left column and normal pancreas at the right column. Pancreatic cancer samples revealing a faint protein band at 46 kD representing KLFwt and bands at 26 kD, 30 kD, and 40 kD representing known forms of KLF6. In contrast, KLF6 protein expression in normal pancreas showing only one strong band at 46 kD representing KLF6wt.

Article Snippet: The sections were then incubated overnight at 4°C with rabbit polyclonal antibody KLF6 (sc7158, Santa Cruz Biotechnology, Santa Cruz, CA) diluted in 0.05 mol/L Tris-HCL buffer containing 1% bovine serum albumin.

Techniques: Western Blot, Expressing

Journal: European journal of cancer (Oxford, England : 1990)

Article Title: Increased alternative splicing of the KLF6 tumor suppressor gene correlates with prognosis and tumor grade in patients with pancreatic cancer

doi: 10.1016/j.ejca.2008.06.030

Figure Lengend Snippet: LOH of the KLF6 and p53 locus was analyzed in tumor tissue from seven patients using microsatellite markers (vertical axis, with patient # above each axis) from the 10p15 region and KLF6-specific markers KLF6M1, M2 and M4. These markers flank the KLF6 gene by approximately 40 Kb centromerically, 10 Kb and 20 Kb telomerically. The lower half displays microsatellite markers flanking the p53 gene. Black filled circle - LOH; gray - non-informative (NI); white circle - no evidence of loss.

Article Snippet: The sections were then incubated overnight at 4°C with rabbit polyclonal antibody KLF6 (sc7158, Santa Cruz Biotechnology, Santa Cruz, CA) diluted in 0.05 mol/L Tris-HCL buffer containing 1% bovine serum albumin.

Techniques: